Abstract

A process for the synthesis of a glycoside, comprising the step of; performing hydrolysis of a methyl ester glycoside in the presence of a base in water, to form a glycoside, wherein the propensity to reform the methyl ester glycoside is reduced. The invention also extends to methods for the detection or prognosis of cancer using a glycoside obtained by said process.

IPC Classes  ?

• C07H 15/04 - Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of a saccharide radical
• C07H 1/00 - Processes for the preparation of sugar derivatives
• C07H 15/00 - Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals

Abstract

A method for the early detection and monitoring of the progression of cancer by detecting breath biomarkers is provided. The method comprises assessing the activity of an aldo-keto reductase by measuring the concentration of an exogenous substrate for said enzyme and/or measuring the concentration of a metabolite of said substrate in exhaled breath of a subject. Preferably, the cancer is lung cancer.

IPC Classes  ?

• C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
• A61B 5/08 - Measuring devices for evaluating the respiratory organs
• A61K 51/04 - Organic compounds
• G01N 33/497 - Physical analysis of biological material of gaseous biological material, e.g. breath
• G01N 33/574 - Immunoassay; Biospecific binding assay; Materials therefor for cancer